Reversed Phase Analysis

Polymeric Spheres

Concise Separation’s pure polymer synthesis technique gives rise to exceptional reversed phase columns.  No need for shielding or extravagant coatings as these highly cross linked spheres are pH stable from 0 to 14….Forever.

Benefits

  • High Temperature Stability
  • Incredibly Clean Spheres: 99.999% Carbon
  • Tight bead size ranges

Column

Bead Size

Phase

Porosity

Dimensions

ProteinSep

2µm

C18

Non Porous

4.6x50mm

PRX-1

5µm

Neutral, non alkylated

100Å

3.2x150mm, 4.6x150mm, 4.6x250mm, 100×4.6mm

OligoSep HC

5µm

C18

100Å

7.8x50mm

OligoPrep HC3

10µm

C18

100Å

7.8x150mm

Silica Spheres

Most HPLC manufacturers offer a silica line of columns.  We only offer Unique phases for those who need better separation than what is commercially available.  We are proud to announce our new line of RPSep Silica Columns.

Column

Bead Size

Phase

Porosity

Dimensions

RPSep HQC18

3µm or 5µm

Unique C18

100Å

2.1x 50 or 100mm

4.6x 50 or 100mm

RPSep ARo

3µm or 5µm

Unique C18

Non Porous

2.1x 50 or 100mm

4.6x 50 or 100mm

RPSep Fluoro

3µm or 5µm

Pentafluorophenylpropyl

Non Porous

2.1x 50 or 100mm

4.6x 50 or 100mm

RPSep HQC18 Column

Benefits

  • LC-MS Compatible
  • Excellent Carbon Loading
  • Type B Spherical Silica, 100 Pores
  • USP L1 Compliant
  • Highest Purity and Column to Column Reproducibility

Uses

  • Traditional Reversed Phase Analysis
  • Excellent for Acidic, Neutral & Basic Compounds

RPSep ARo Column

Benefits

  • LC-MS Compatible
  • Special Selectivity compared to Normal C18 phase
  • Significant Selectivity Changes between Methanol & Acetonitrile

Uses

  • Clinical & Forensics, Research & Method Development
  • Strong interaction with Aromatics
  • Drugs of Abuse
    • Buprenorphine, Clenbuterol, Diazepam, EDDP, Gabapentin, Methadone, Nobuprenorphine, Oxazepam, PCP, Salbutamol, THC

RPSep Fluoro Column

Benefits

  • LC-MS Compatible
  • Can be used with Reversed or Normal Phase and HILIC Mode
  • Special Selectivity compared to Normal C18 phase

Uses

  • Pharmaceuticals, natural products, research & method development
  • Strong interaction with Basic, Halogenated Analytes and Aromatics, such as Fluorines and Amines
    • Beta Blockers, Fluorinated Corticosteroids, Nucleosides, Nucleotides, Purines, Pyrimidines, Taxanes and Tricyclic Antidepressants

Other Focuses